Soft tissue shortage after oral and neck surgery is common. Split thickness skin graft and synthetic dermal graft are the gold standards for raw surface repair now. However these graft materials are not abundant, nor bio-controlled. Ex-vivo soft tissue engineering is an emerging science not only to fulfill soft tissue coverage but also to explore prosperous stem cell applications.
Autogenous oral keratinocytes cultured in serum-free medium provide an exogenous-pathogen -free cell resource and AlloDerm, a dermal allograft, give a robust basement membrane anchorage and three dimensional framework. Chemical defined serum-free medium furnishes the ex-vivo engineered soft tissue.
Dr. Wen Liang Lo, DDS, PhD
Assistant Professor, National Tang-Ming Medical University